bs-2499R [Primary Antibody]
CD69 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD69/CLEC2C/AIM

Immunogen Range: 100-150/199

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 969

Swiss Prot: Q07108

Source: KLH conjugated synthetic peptide derived from human CD69

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69/CLEC2C/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69/CLEC2C/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: mouse intestine lysates Lane 2: mouse lung lysates probed with Anti CD69/CLEC2C/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 22kD. Observed band size: 22kD.


Mouse spleen lysates probed with Rabbit Anti-CD69 Polyclonal Antibody, Unconjugated (bs-2499R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.