bs-1329R [Primary Antibody]
ZO-1 Polyclonal Antibody
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Host: Rabbit

Target Protein: ZO-1

Immunogen Range: 1670-1720/1733

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7082

Swiss Prot: Q07157

Source: KLH conjugated synthetic peptide derived from human ZO-1

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

293T cells probed with ZO-1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-1329R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).


Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining


Image was kindly submitted by Sriram Srivatsa from Medical University of Vienna.Paraffin-embedded mouse colon stained with Rabbit Anti-ZO-1 Polyclonal Antibody (bs-1329R)at 1:100.


Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.


Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.