bs-1035R [Primary Antibody]
CD86 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD86

Immunogen Range: 130-180/313

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 56822

Swiss Prot: O35531

Source: KLH conjugated synthetic peptide derived from rat CD86

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with Anti-CD86/B7-2 Polyclonal Antibody (bs-1035R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat lung labeled with Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


L1 rat brain lysates L2 rat heart lysates probed with Anti CD86/B7-2 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD. Observed band size:34kD.


HeLa cell lysates probed with Rabbit Anti-CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.