bs-0647R [Primary Antibody]
CD4 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD4

Immunogen Range: 420-457/457

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24932

Swiss Prot: P05540

Source: KLH conjugated synthetic peptide derived from rat CD4

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, AF555 conjugated (bs-0647R-A555) at 1:200 at 4°C for 90 minutes and DAPI staining of the nuclei.


Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.


Image provided by the Independent Validation Program, badge number 029634:Formalin-fixed and paraffin embedded human kidney labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 followed by conjugation to the secondary antibody and DAB staining


Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with (bs-0647R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.